FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_3694_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_3694_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15130911
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGATGTGCTGTGTAGATCTCGGTGGTCGCCGTATCATTA10204046.743837168826121Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGATGTGCTGTGTAGATCTCGGTGGTCGCCGTATCATTT195130.12896117094337545Illumina Single End PCR Primer 1 (96% over 30bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGAAGGATGTGCTGTGTAGATCTCGGTGGTCGCCGTATCATTA166580.11009251194458813Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)