FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_3688_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_3688_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15617682
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGACGTGTACCAGGTGTAGATCTCGGTGGTCGCCGTATCATTA5886923.7693942033139103Illumina RNA PCR Primer (96% over 31bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGACGTGTACCAGGTGTAGATCTCGGTGGTCGCCGTATCATTAA897630.5747523864296891Illumina RNA PCR Primer (96% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGACGTGTACCAGGTGTAGATCTCGGTGGTCGCCGTATCATTT160630.1028513706451444Illumina RNA PCR Primer (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)