FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n01_67192_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n01_67192_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888682
Sequences flagged as poor quality0
Sequence length74
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA993480.9123969273783549Illumina PCR Primer Index 7 (96% over 27bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATCTCGTATGCCGGCTTCTGCTTGAAAAA350620.32200407726114144Illumina PCR Primer Index 3 (96% over 25bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATGTCGTATGCCGTCTTCTGCTTGAAAAA211360.19410981053537976Illumina PCR Primer Index 3 (96% over 25bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATCTCGTATGCCGTCTTCTGCGTGAAAAA169530.15569377450824629Illumina PCR Primer Index 3 (96% over 25bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATCTCGTATGCCGGCTTCTGCGTGAAAAA156910.14410375837957248Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTCTGTCATGTCGTATGCCGGCTTCTGCTTGAAAAA151340.13898835506446053Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)