FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n01_3694_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n01_3694_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14876892
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCTTCTGCTTGAAAAA5571093.7447942755785277RNA PCR Primer, Index 29 (96% over 28bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCTTCTGCTTGAAAAAA1557681.0470466546372725RNA PCR Primer, Index 29 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCTTCTGCGTGAAAAA1133950.7622223781687735Illumina PCR Primer Index 12 (96% over 26bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAG491620.33045880819730356No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCGTCTGCGTGAAAAA266850.17937214305246016Illumina PCR Primer Index 12 (95% over 23bp)
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCTTCTGCTTGAAAAA215600.1449227432719146RNA PCR Primer, Index 29 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCGTCTGCTTGAAAAA207360.1393839519706132Illumina PCR Primer Index 12 (96% over 26bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGAGGAATAATCTCGTATGCCGTCTTCTGCGTGAAAAAA154970.10416826310226625Illumina PCR Primer Index 12 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)