FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n01_3694_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n01_3694_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15130911
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAATTGTAAATCTCGTATGCCGTCTTCTGCTTGAAAAA5616053.7116403632273034RNA PCR Primer, Index 15 (96% over 29bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAATTGTAAATCTCGTATGCCGTCTTCTGCGTGAAAAA892410.5897926436815337RNA PCR Primer, Index 15 (96% over 26bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAATTGTAAATCTCGTATGCCGTCGTCTGCTTGAAAAA181130.11970858859721005RNA PCR Primer, Index 29 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)