FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n01_3688_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n01_3688_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18383879
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGTGGCACTATCTCGTATGCCGTCTTCTGCTTGAAAAA854540.4648311708317924Illumina PCR Primer Index 1 (96% over 28bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGTGGCACTATCTCGTATGCCGGCTTCTGCTTGAAAAA394300.2144813942694031RNA PCR Primer, Index 27 (96% over 26bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGTGGCACTATCTCGTATGCCGGCTTCTGCGTGAAAAA196740.10701767564941002RNA PCR Primer, Index 27 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)