FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n01_3688_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n01_3688_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16767091
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCTGGATAATCTCGTATGCCGTCTTCTGCTTGAAAAA1728511.0308943871062668Illumina PCR Primer Index 12 (96% over 27bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCTGGATAATCTCGTATGCCGTCTTCTGCGTGAAAAA509590.30392272577276525Illumina PCR Primer Index 4 (96% over 25bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCTGGATAATCTCGTATGCCGGCTTCTGCTTGAAAAA236570.14109185666136123Illumina PCR Primer Index 4 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)