FastQCFastQC Report
Sat 24 Jun 2023
HJHV7AFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHV7AFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692242
Sequences flagged as poor quality0
Sequence length74
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGAT68690.18603872660567752Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40890.11074572035094125Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCG40470.10960820011256033Clontech Universal Primer Mix Long (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTGAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37350.10115804977030216Clontech Universal Primer Mix Long (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)