FastQCFastQC Report
Sat 24 Jun 2023
HJHV7AFX5_n01_CONTROL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHV7AFX5_n01_CONTROL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78197187
Sequences flagged as poor quality0
Sequence length74
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGAT4476470.5724592113524494Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCG2770690.35432092972858475Clontech Universal Primer Mix Long (100% over 21bp)
GTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAG1985000.2538454484302613No Hit
GCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCA1314840.16814415587609308No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGT1225260.15668850082804128Clontech Universal Primer Mix Long (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATC1205910.15421398726273874Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATAT1117060.14285168595642705Clontech Universal Primer Mix Long (100% over 23bp)
AGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1077590.13780418981056186No Hit
CAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGG939050.12008743997402362No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC915720.11710395669348056Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG895350.11449900365341786Clontech Universal Primer Mix Long (100% over 23bp)
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCC866430.11080066089845407No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA826160.10565085928218876Clontech Universal Primer Mix Long (100% over 21bp)
CCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGC821700.10508050628470816No Hit
GGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTA808720.10342060002746646No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG796850.10190264261040488Clontech Universal Primer Mix Long (100% over 21bp)
GTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGT791510.1012197535954842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)