FastQCFastQC Report
Sat 24 Jun 2023
HJHV7AFX5_n01_CAS_TREAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHV7AFX5_n01_CAS_TREAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54006463
Sequences flagged as poor quality0
Sequence length74
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGGCGGGGTACAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGG2900420.5370505378217418Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC2557360.4735285108376751Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGC2397330.4438968721206571Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTGGGGAGTTTG1322660.2449077252105919Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGT1198580.22193269720329584Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCCGCTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAA1086830.20124072928086406Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAA1082380.20041675382444507Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGT1075330.19911135450584866Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCG1012230.1874275676968514Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGAGGCGGGGTACAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGCG952740.17641221940418503Clontech Universal Primer Mix Long (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATTTTAAGAAAAAGAAAGACGAAGCTTCGTTCCAAAGTGTTCAC875550.16211948558823414Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTC830440.1537667815794565Clontech Universal Primer Mix Long (100% over 21bp)
GCAGTGGTATCAACGCAGAGTGAATGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA828700.1534445979178455Clontech Universal Primer Mix Long (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTG808500.14970430483477504Clontech Universal Primer Mix Long (100% over 23bp)
GTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAATCT699550.1295307933793035No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTA630730.11678787407351598Clontech Universal Primer Mix Long (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTGAATGGGAGGCGGGGTACAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGC629130.11649161323525298Clontech Universal Primer Mix Long (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTGAATGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACC570690.10567068611769669Clontech Universal Primer Mix Long (100% over 22bp)
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAA543210.10058240622052957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)