FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah9003i3h.35100000086fdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah9003i3h.35100000086fdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682041
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC17630.10481314070227778TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3150.022.8565711
GATCGGA3450.022.1911681
CACACGT3400.021.1759412
TATCTCG3550.020.91496839
ACGTCTG3750.019.79950315
TAGCTTA4100.019.75560234
CACGTCT3900.019.03798314
CGTCTGA4150.018.97542816
ACACGTC3900.018.46107513
TCGGAAG4300.018.3145833
GTATGCC3950.018.2273945
ACTAGCT4400.017.8972832
CACTAGC4400.017.8972831
CAGTCAC4300.017.7902527
GAACTCC4250.017.47014821
GTCTGAA4650.017.41891717
ATCTCGT4350.017.06853740
AGCACAC4350.016.5513110
CGTATGC4350.016.5513144
CCAGTCA4450.016.17936926
Produced by FastQC (version 0.11.2)