FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah9003c3h.351000000870a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah9003c3h.351000000870a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072210
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG26470.12773801883013786TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC4700.024.41435829
ACGTCTG5000.023.84947415
GATCGGA4850.023.6718391
GCACACG4800.023.43698511
ACACGTC5300.022.07498713
CACGTCT5450.021.88025314
TCCAGTC5600.020.49062225
CACACGT6000.019.87456312
CGTCTGA5900.019.83007216
GAACTCC5900.019.06737721
TATCTCG5850.018.07652541
AGCACAC6600.018.06778510
CACACTG6000.017.99960531
ACTCCAG6250.017.99960523
ATCTCGT5950.017.77271842
CCAGTCA6500.017.30731226
CAGTCAC6450.017.0926527
TCACACT6650.016.91692230
TCGGAAG7050.016.9157473
GAGCACA6900.016.303999
Produced by FastQC (version 0.11.2)