FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797i3h.35100000086fb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797i3h.35100000086fb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765197
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC47880.2712445126521289TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7000.035.3834271
GCACACG7350.033.67272611
CGATCAG7450.033.5227533
ACACGTC7500.032.39928413
CACGATC7700.032.14214731
AGTCACG7800.031.44161628
ACGTCTG7900.031.0436215
CACGTCT7900.030.75881614
GTCACGA8150.030.3674329
AGCACAC8400.029.46363810
GAGCACA8350.029.3706119
CACACGT8350.029.10115612
AGAGCAC8600.029.0400568
GATCTCG8550.028.68357739
GTATGCC8600.028.51681145
CGTCTGA8950.028.40719416
CAGTCAC9000.027.99938227
GAACTCC8850.027.71125421
CGTATGC8900.027.55557344
CCAGTCA8900.027.55557326
Produced by FastQC (version 0.11.2)