FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797i34c3h.35100000086f49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797i34c3h.35100000086f49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624827
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3000.022.514121
GCACACG3250.020.76883111
ACGTCTG3550.019.64751215
CGATCTC3600.018.74964138
CACACGT3850.018.11653712
ACACGTC3900.017.88427413
CACGATC4200.017.67823236
CACGTCT4200.017.67823214
CATCACG3950.017.65788833
AGTCACA3900.017.30736228
GTCACAT3800.017.17072529
TCGGAAG4100.017.0123943
GATCTCG4150.016.80690839
GAGCACA4250.016.4114519
CGTATGC4550.016.31836744
CAGTCAC4000.016.31218727
AGCACAC4300.016.2206210
CGTCTGA4500.015.99969316
ATCACGA4650.015.96743734
TCCAGTC4300.015.69737325
Produced by FastQC (version 0.11.2)