FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797i0h.35100000086f99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797i0h.35100000086f99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890138
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC26890.1422647446905993TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG4600.027.87994211
GATCGGA4850.026.4575311
GTCACAC5150.026.2131329
CAGTCAC5200.024.23034127
AGAGCAC5550.023.9184978
GAGCACA5650.023.4951619
CACACGT5750.023.47784812
CACGTCT5600.023.30316214
ACACGTC5600.022.90138413
GTATGCC5950.022.31112145
CGTCTGA5950.021.93238416
ACGTCTG6150.020.8532915
CGTATGC6300.020.7139244
AGCACAC6400.020.39026610
CCAGTCA6300.020.35678526
TCGTATG6500.019.73042343
AGTCACA6650.019.62371328
TCCAGTC6750.019.33299325
GAACTCC6900.018.9127121
GTCTGAA7200.018.7496717
Produced by FastQC (version 0.11.2)