FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797c3h.3510000008707b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797c3h.3510000008707b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007143
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG36770.18319571649852553TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5550.031.2373491
CACGAGT6900.024.7820931
TCACGAG7150.024.54493730
AGTCACG7000.024.42805928
ACGAGTG7100.024.40089632
CGAGTGG7250.023.8960533
GTCACGA7200.023.749529
ACGTCTG7600.023.68371215
ACACGTC7650.023.52891713
GCACACG7300.023.42416611
CACACGT7950.022.07500612
GAGCACA7950.021.7919949
CACGTCT8000.021.3745514
CGTCTGA8450.021.30132716
TATCTCG8450.021.0350641
ATCTCGT8350.021.0175242
CTCGTAT8450.020.50303844
CAGTCAC8450.020.50252727
AGAGCAC8450.020.5025278
AGCACAC8750.019.79958310
Produced by FastQC (version 0.11.2)