FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797c34c3h.3510000008701e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797c34c3h.3510000008701e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854986
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG56420.304153238892369TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7500.037.49894311
AGTCACG7700.035.35614828
CACACGT8100.034.44347812
ACACGTC8150.034.23216613
AGCACAC8350.033.9511410
CACGTCT8250.033.5445114
CGTGGCC8400.032.409833
ACGTCTG8850.032.03299715
CACGTGG8650.031.99331931
TCACGTG8800.031.70365330
GATCGGA8800.031.471731
CCAGTCA8950.031.17230626
ACGTGGC8900.031.0946332
GTCACGT8850.031.01607729
TCCAGTC8900.030.84182725
CAGTCAC8900.030.84182727
GAGCACA9300.030.7249439
GAACTCC9050.030.08202621
TCGGAAG9350.030.08183
GGCCTTA9200.030.08067536
Produced by FastQC (version 0.11.2)