FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7797c0h.35100000087052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7797c0h.35100000087052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982822
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG23370.1178623194618579TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3650.029.6100831
ACGTCTG4600.023.96689615
GCACACG4750.023.21004711
CGTCTGA4950.023.18133716
ACACGTC4950.022.726813
CCGTACG4650.022.25760333
CACGTCT4900.022.0403614
CGTACGT4850.020.4119534
CACACGT5500.020.0450412
CAGTCAC5300.019.95241527
AGAGCAC5650.019.911098
ACCGTAC5400.019.58292832
CCAGTCA5550.019.45905726
AGCACAC5800.019.00822610
ATCTCGT5500.018.4087142
CGTAATC5650.018.31820338
TCCAGTC6050.018.22276325
ACTCCAG6050.018.22276323
GAGCACA6150.017.9264589
GTCACCG5900.017.92335529
Produced by FastQC (version 0.11.2)