FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7669i3h.35100000086ff6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7669i3h.35100000086ff6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656658
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2850.027.63096811
TCACGGC3500.026.35655830
CACGGCT3400.024.48475331
AGTCACG3500.023.78518728
GTCACGG3550.023.45018429
CGGCTAC3700.022.499533
GATCGGA3650.022.2067051
CGTATGC4100.020.30442844
ACGGCTA4150.020.05979732
CACACGT4050.019.99955712
GTATGCC4100.019.75566145
ACACGTC4000.019.68706513
AGCACAC4250.019.05840110
TCCAGTC4550.018.79079425
ACGTCTG4450.018.7074515
CATCTCG4350.018.62027739
CAGTCAC4600.018.58654627
CGTCTGA4400.018.40868416
TCGTATG4800.017.81210543
GAACTCC4550.017.80180521