FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7669c3h.351000000870cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7669c3h.351000000870cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593030
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG19840.12454253843304897TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2650.031.41419811
CACACGT3500.024.42787412
ACACGTC3450.024.12974513
TATCTCG3450.024.12974541
GATCGGA3600.023.7634931
AGCACAC3650.023.42398610
CACGTCT3700.023.10744914
TCCAGTC3800.022.49935725
GAGCACA4100.020.3042989
ACGTCTG4300.019.88315215
CTCGTAT4250.019.58767544
CCAGTCA4550.019.28516426
CAGTCAC4750.018.47315627
TCTCGTA4450.018.20172543
AGTCACA4950.018.18129728
GAACTCC4600.018.09730721
CGTCTGA4750.017.99948516
TCGTATG4900.017.4484845
ATCTCGT4650.016.93542
GTCACAT4950.016.817729
Produced by FastQC (version 0.11.2)