FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7606i34c3h.35100000086f62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7606i34c3h.35100000086f62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037932
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC22800.11187811958397041TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3700.024.32375311
CTTAGGC4000.022.49947433
GATCGGA4250.021.1884521
ACGTCTG4600.020.05387915
ACTTAGG4400.019.94271532
CACGTCT4650.019.83824314
CACACGT4750.018.94692212
CGTCTGA5150.018.78596716
CATCTCG4750.018.4732539
ACACGTC4950.018.18139313
AGCACAC5500.017.59049810
CAGTCAC5750.016.82569327
CCAGTCA5550.016.62123326
AGTCACT5950.015.88198128
ATCTCGT5650.015.53060840
CACTTAG5800.015.51687831
GTATGCC5550.015.40504545
GAACTCC6000.015.374639521
TCCAGTC5900.015.2538825
GAGCACA6150.014.9996489
Produced by FastQC (version 0.11.2)