FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah7606c34c3h.35100000087038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah7606c34c3h.35100000087038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887619
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG2000.024.74934428
GTCACGT2500.020.69945329
ACGTCTG2650.020.37681815
CACGTCT2750.019.63584314
GCACACG2600.019.03795811
CGTTTCG3300.017.04500433
ACACGTC3050.016.96676413
CACACGT3000.016.49956312
GATCGGA3300.016.3749141
CGTCTGA4050.014.44406216
CTCGTAT3750.014.39961944
GTTTCGG3450.014.34744634
CAGTCAC3300.014.31780327
GAGCACA4150.013.5538589
AGAGCAC4200.012.8568028
AGCACAC4150.011.92739510
GAACTCC4550.011.86781721
ATCTCGT4650.011.61259642
TCTCGTA4550.011.37332443
TCACGTT5000.010.79971430