FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah5187i3h.3510000008711d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah5187i3h.3510000008711d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567637
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG21500.13714909765462285TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG4350.028.96471611
CACACGT4550.027.19705212
ACACGTC4650.027.09602513
ACGTCTG4750.026.52558115
CACGTCT5000.025.64929214
GATCGGA4950.025.0184611
TCCAGTC5100.024.70520225
CGTCTGA5050.024.50427416
AGCACAC5250.023.57077610
GAACTCC5500.022.90845921
TCGGAAG5850.022.3084983
ATCTCGT5650.022.3002742
CAGTCAC5500.022.090327
CCAGTCA5600.021.6958326
ACTCCAG5750.021.52114323
GTCACAT5800.021.33561729
TCTCGTA6000.020.6244343
AGTCACA6200.020.32201828
GAGCACA6350.020.1962939
CTCGTAT6000.019.8744544
Produced by FastQC (version 0.11.2)