FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah5187c3h.35100000087187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah5187c3h.35100000087187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224156
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1982808.914842304226863TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGC94520.4249701909398442TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA226250.044.5747531
TCGGAAG240000.041.926153
GCACACG236150.041.8550811
AGAGCAC239000.041.7419478
AGCACAC237550.041.71259310
ATCGGAA242100.041.70472
CACACGT237200.041.66031612
ACGTCTG235750.041.63978215
GAGCACA238200.041.6271069
ACACGTC236700.041.62474413
CGTCTGA236850.041.60788316
CACGTCT236450.041.59263614
GAACTCC235250.041.39353621
CGGAAGA242900.041.387614
TCCAGTC234600.041.3068225
AGTCACG236550.041.289728
CAGTCAC235900.041.24133327
TCGTATG234800.041.23423445
GAAGAGC242900.041.2208756
GTCTGAA239750.041.1702917
Produced by FastQC (version 0.11.2)