FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah5184i3h.35100000087100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah5184i3h.35100000087100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237448
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG170790.7633250024134639TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23500.038.9914971
GCACACG24850.036.21638511
ACACGTC25500.035.29322413
ACGTCTG25700.035.0185715
CACACGT25700.035.0185712
CCGTATC25200.034.99911538
CGTCTGA25900.034.83502616
AGCACAC26100.034.74049810
CACGTCT26300.034.30521414
GAACTCC26400.033.91959421
TATCTCG25950.033.7274741
AGTTCCG26600.033.6645634
GAGCACA27050.033.5204059
TCCAGTC26500.033.45198425
CCAGTCA26800.033.4133326
GTCACAG26650.033.34812529
CTCGTAT26850.033.26731544
CGTATCT26550.033.13475439
TCGGAAG28000.032.8681763
CAGTCAC27250.032.6964227
Produced by FastQC (version 0.11.2)