FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah5184c3h.35100000087151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah5184c3h.35100000087151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304008
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG283551.2306814906892685TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC70370.3054242867212267TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35000.041.3633231
ACGTCCG41200.033.803732
GCACACG41500.033.61355211
ACGTCTG41550.033.57309715
CACGTCT41650.033.2764114
ACACGTC42200.033.05597713
AGTCACG42200.032.94934528
TCACGTC42150.032.93505530
AGAGCAC43000.032.91198
AGCACAC42400.032.90005510
CGTCCGC42250.032.857133
TCGGAAG44200.032.7847633
GAGCACA42800.032.7502869
GAACTCC42950.032.74067321
CGTCTGA43150.032.64106816
CACACGT42900.032.621512
CAGTCAC42550.032.51968827
CACGTCC42650.032.49619331
GTCCGCA42900.032.4641634
TCCAGTC43050.032.40330525
Produced by FastQC (version 0.11.2)