FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah119i3h.351000000870e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah119i3h.351000000870e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662254
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG21510.12940260634054723TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4300.027.7317413
GATCGGA4700.026.3480321
AGCACAC4900.024.7951910
GCACACG4750.024.63085611
ACGTCTG4950.024.54473315
CAGTCAC4950.024.54473327
CACACGT4900.024.33602112
CACAGTC5100.024.26399231
CGTCTGA5050.024.05869716
CACGTCT5150.023.5915414
CCAGTCA5350.023.13016126
AGTCACA5400.022.91599328
TCGTATG5450.022.70643645
ATCTCGT5600.022.49933842
GAGCACA5400.022.4993389
GAACTCC5650.022.3002321
CTCGTAT5350.022.28906444
TCCAGTC5550.021.89124925
GTCACAG5650.021.50379229
AGAGCAC5750.020.7385228
Produced by FastQC (version 0.11.2)