FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l02n01_ah119c3.35100000087144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l02n01_ah119c3.35100000087144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926576
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG361871.878306383968242TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41700.043.64999811
GTCCCGA40650.043.55980336
ACACGTC41800.043.43791613
TCGGAAG42250.043.404673
CGTCCCG40850.043.2363835
GATCGGA42700.043.2354131
GAGCACA42000.043.231079
CACGTCT42000.043.177514
CACACGT42100.043.12838412
ACCCGTC41400.043.04240832
ACGTCTG42050.042.9656415
CCCGTCC41550.042.88702433
CACCCGT41700.042.7867131
ATCGGAA43300.042.720332
CAGTCAC41800.042.68434527
AGCACAC42650.042.67772310
TCGTATG41450.042.66480345
TCCCGAT41300.042.65632637
AGTCACC42050.042.64460428
CGATCTC41350.042.387140