FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9120red1-032817.3510000008706e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9120red1-032817.3510000008706e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195462
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1850742.987250991128668TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG212850.043.8357411
ACACGTC213650.043.51363413
CACACGT214500.043.4670712
AGCACAC215900.043.3102710
CACGTCT213700.043.2823614
GAGCACA216400.043.2101949
ACGTCTG213650.043.2082415
AGTCACG212100.043.0388228
GATCGGA218350.043.0227361
GTCACGT212650.042.9800629
TCACGTG213250.042.89078530
CACGTGA213000.042.88830631
TCGGAAG219000.042.8629573
GAACTCC215850.042.47633421
CAGTCAC214450.042.4727627
CGTCTGA217200.042.46093816
CGATCTC211050.042.44165440
AGAGCAC221050.042.352128
TCGTATG214450.042.33362245
ATCGGAA222650.042.3017652
Produced by FastQC (version 0.11.2)