FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9120i-032817.35100000087001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9120i-032817.35100000087001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12407298
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG127990.10315702903242914TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24050.026.1010911
ACACGTC26800.023.92652313
GATCGGA27450.022.9630891
CACACGT27800.022.58026112
ACGTCTG28500.022.10460315
CACGTCT28900.021.79865614
CGTCTGA30950.020.28218816
AGCACAC32600.019.73867410
GAGCACA33250.019.4881389
CAGTCAC32850.019.38391927
TCCAGTC33950.018.68959425
CCAGTCA34750.018.1945826
GAACTCC35100.017.94825421
AGTCACA36500.017.8770428
AGAGCAC37100.017.526438
CACATGT36650.017.37405831
GTCACAT37650.017.27116629
ATCTCGT35950.017.14844342
TCACATG36800.017.11981230
CTCGTAT37450.016.8822144
Produced by FastQC (version 0.11.2)