FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9041red1-032817.35100000087045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9041red1-032817.35100000087045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761439
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG480090.7100411613563327TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57700.040.98252511
ACGTCCG56500.040.77775632
GATCGGA59900.040.5115621
CGTCCGC56700.040.43551333
ACACGTC59000.039.92698313
AGTCACG58650.039.70696328
CACACGT60050.039.34124412
CCGCACA58000.039.29818736
ACGTCTG61050.038.88110415
GTCACGT59850.038.87323429
CACGTCT60750.038.81385814
CACGTCC59650.038.66408531
TCACGTC60150.038.60453830
AGCACAC62000.038.24905410
TCCGCAC59650.038.2488735
GTCCGCA60200.038.16020234
CGTCTGA62400.038.14809816
CATCTCG60400.037.77392641
GAGCACA64200.037.1132979
CGCACAT61900.037.0403137
Produced by FastQC (version 0.11.2)