FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9041i-032817.35100000086fe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9041i-032817.35100000086fe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930982
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG444870.5609267553500941TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA57100.038.7538761
GCACACG56100.038.7424811
CACACGT57650.037.89597312
ACGTCTG58350.037.4799115
ACACGTC58450.037.37729313
CACGTCT59300.036.84153414
CGTCTGA59800.036.53349316
CCGTATC59050.036.16016838
TATCTCG59150.036.13707441
AGCACAC60750.036.11033210
AGTTCCG60450.035.50814434
GAGCACA62100.035.3253259
AGAGCAC62550.035.17918
GTTCCGT61200.035.1471935
GAACTCC62950.034.7768521
CTCGTAT62000.034.5476444
GTCACAG62800.034.5383429
TCCAGTC62950.034.420325
CCAGTCA63000.034.3572726
GTCTGAA63900.034.22461317
Produced by FastQC (version 0.11.2)