FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9003i-032817.35100000086f3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9003i-032817.35100000086f3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7828722
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC97250.124222063320169TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17700.027.96532211
ACGTCTG18950.025.64572315
ACACGTC19800.025.1129413
CACACGT19800.025.1129412
CACGTCT19950.024.9241214
CGTCTGA20300.023.9402216
ACTGACC21150.023.19099232
GTATGCC21550.022.65627145
GATCGGA22000.022.4089811
CAGTCAC22350.021.9462627
CCAGTCA22950.021.37250126
TCCAGTC23050.021.27977825
CGTATGC23350.021.10219644
AGCACAC24150.020.86898610
GAGCACA24700.020.2221099
CTCGTAT25300.019.38681242
AGTCACT26250.018.77138528
TCTCGTA26700.018.62343441
TCGTATG26950.018.53380843
AGAGCAC27250.018.49498
Produced by FastQC (version 0.11.2)