FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9003h3k79-032817.35100000086fa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9003h3k79-032817.35100000086fa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465443
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1465261.9627234445430766TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG167750.043.10774611
GATCGGA170700.042.7309381
ACACGTC169050.042.709713
CACACGT169400.042.67458712
ACGTCTG170700.042.2971515
CACGTCT170950.042.2218514
AGCACAC172950.041.9157210
CGTCTGA172550.041.83033816
GAGCACA173950.041.7523659
GTATGCC171850.041.43774845
AGAGCAC175850.041.4033248
TCGGAAG176950.041.3773083
CAGTCAC173200.041.37568727
CGTATGC172850.041.2758444
GAACTCC175300.041.10995521
TCCAGTC175350.040.95818725
AGTCACC175400.040.85671628
GTCACCT175600.040.82299829
CCAGTCA176000.040.79413226
CTCGTAT174850.040.62356642
Produced by FastQC (version 0.11.2)