FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9003h3-032817.35100000086f7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9003h3-032817.35100000086f7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7853630
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC727590.9264378382989777TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC86900.041.5573829
GCACACG87850.041.5412811
ACACGTC89300.040.8415613
TCACGCC88400.040.8267730
AGTCACG90350.040.36923628
CACACGT90550.040.3523112
GATCGGA92750.039.997221
CACGCCA90400.039.87374531
AGCACAC92450.039.76636510
CGTCTGA92400.039.64178516
ACGTCTG92550.039.55322615
CACGTCT92600.039.45897714
TATCTCG92100.039.40622739
GAACTCC93150.039.08082221
GTATGCC92350.039.02955645
GAGCACA94900.038.810619
AGAGCAC96450.038.2802168
TCCAGTC96150.037.7697725
CAGTCAC97000.037.6243727
ATCTCGT96350.037.3875540
Produced by FastQC (version 0.11.2)