FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9003-3-032817.351000000870d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9003-3-032817.351000000870d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959327
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC600680.43030727770758576TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG66250.041.1276611
CACACGT70250.038.94601412
CGATCAG71250.038.2415133
GATCGGA72850.038.006941
ACACGTC72750.037.48394813
GTATGCC73850.036.65168845
GAGCACA75600.036.5470479
GAACTCC74850.036.46235721
AGTCACG75250.036.32898728
ACTCCAG75700.036.23140323
ACGTCTG75750.035.99943515
CAGTCAC76200.035.75795727
AGCACAC77500.035.5349310
GTCACGA76950.035.43842729
CGTATGC77500.035.0413944
AGAGCAC80100.034.662268
GATCTCG78800.034.52052339
CACGTCT79650.034.2367514
ACGATCA80500.033.81934732
CGTCTGA82400.033.0395316
Produced by FastQC (version 0.11.2)