FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_9003-0-032817.351000000870be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_9003-0-032817.351000000870be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838787
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1684704.388625886250005TruSeq Adapter, Index 8 (100% over 51bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC46940.12227820923640723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA179400.044.5236781
GCACACG178150.044.46822411
CACACGT179200.044.27044312
ACGTCTG179800.044.0851715
ACACGTC179950.044.0359213
GAACTCC179650.044.03431321
GAGCACA180800.043.953349
GTCACAC179300.043.9463129
AGCACAC180950.043.86716510
GTATGCC178200.043.73720645
ACTCCAG180350.043.68874423
CACGTCT181350.043.64634314
AGAGCAC182950.043.47378
CGTCTGA182500.043.45760716
TCACACT181850.043.39193330
CAGTCAC181200.043.32407427
CGTATGC180900.043.18335744
AGTCACA182800.043.0925828
CTGAACT184350.043.0825219
ATCGGAA185200.043.0559962
Produced by FastQC (version 0.11.2)