FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_8643red1-032817.3510000008702b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_8643red1-032817.3510000008702b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794478
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG896001.149531758252445TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG97600.042.8339935
GTCCCGA98300.042.59763336
GCACACG102200.042.18081311
CCCGTCC100700.041.46961633
ACCCGTC101350.041.2253232
ACACGTC104700.041.19511813
CCCGATC101550.041.19002538
CACACGT104800.041.1558112
TCACCCG102250.041.14930730
CACCCGT102500.040.91723631
CCGTCCC102050.040.83387434
GATCGGA107150.040.758621
ACGTCTG105950.040.5179715
CACGTCT106850.040.36620714
CCGATCT103900.040.3450239
AGCACAC107250.040.29956410
CGATCTC103800.040.18802340
GTCACCC104750.040.16722529
TCCCGAT104650.040.03437837
GAGCACA108300.040.033259