FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_8643i-032817.35100000086fcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_8643i-032817.35100000086fcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394536
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG100060.1353161307213867TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16700.027.48412111
ACACGTC17950.025.82087513
GATCGGA18600.025.1752661
ACGTCTG18650.024.85172315
CACGTCT19250.024.31088414
CGTCTGA19250.024.1941716
CACACGT19650.023.81600812
AGCACAC20150.023.67167510
GAGCACA21100.022.2859949
CAGTCAC21050.021.91257727
CACAGTC21500.021.767931
TCCAGTC22900.020.0440925
GAACTCC23050.020.0103221
ATCTCGT23050.020.01018342
GTCACAG23600.019.92626829
TCGGAAG24700.019.6757283
AGTCACA23900.019.58213428
CCAGTCA24050.019.3663126
TCTCGTA24150.019.09874743
AGAGCAC24450.019.048458
Produced by FastQC (version 0.11.2)