FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_7797i-032817.35100000086f2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_7797i-032817.35100000086f2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8322749
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC272920.3279204983834067TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36300.037.0649111
ACGTCTG38900.034.52972815
ACACGTC39900.033.83349213
CACACGT39800.033.74890512
CACGTCT40300.033.33018514
CGTCTGA40650.033.09875516
TATCTCG40650.032.49128339
GATCGGA41550.032.2383461
AGCACAC42050.031.99658410
GTATGCC41150.031.98617445
GAGCACA42600.031.5834859
CCGATGT42200.031.24343933
TCCAGTC42500.031.02382725
CCAGTCA43000.030.66308626
GTCACCG43600.030.5507529
CGTATGC43550.030.32622544
CGATGTA43700.030.32619734
GAACTCC44350.030.28668621
CAGTCAC43600.030.24111627
AGAGCAC44650.030.1837928
Produced by FastQC (version 0.11.2)