FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_7797h3k79-032817.35100000086f8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_7797h3k79-032817.35100000086f8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600465
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC354210.41184982439902956TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG47900.038.98571811
GATCGGA48950.038.5377731
CACACGT51100.036.50031312
ACACGTC52000.036.0416413
AGCACAC53500.035.1572910
CGTCTGA53800.034.62689216
ACGTCTG53800.034.6266915
CATCTCG54000.034.16750739
GAGCACA55900.034.090599
CACGTCT55000.034.03483214
GTATGCC54500.033.9348445
GAACTCC55650.033.6374921
AGAGCAC57000.033.274828
CGTATGC56000.033.1462144
TCCAGTC56150.032.94032725
CAGTCAC56650.032.68931227
TCGGAAG60050.031.6980743
CCAGTCA58850.031.6202226
CTCGTAT58850.031.4641742
ATCGGAA60750.031.2957942
Produced by FastQC (version 0.11.2)