FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_7797h3-032817.35100000086f56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_7797h3-032817.35100000086f56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7518959
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1382521.8387119812729396TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG158400.042.71199811
CACACGT158850.042.5343512
ACACGTC158850.042.4635313
GATCGGA162350.042.206631
ACGTCTG160450.041.99801615
CGTCTGA160050.041.99051716
CACGTCT160800.041.94857814
GTCACAC160850.041.67115829
AGCACAC163650.041.5342510
GATCTCG160800.041.45995339
GTATGCC161800.041.28660245
GAGCACA165200.041.2671289
GAACTCC163150.041.23402821
AGAGCAC166700.041.0712628
CACACAG163050.041.06749731
CAGTCAC164150.040.79229427
AGTCACA165150.040.57254428
TCCAGTC165000.040.55488225
AGTGATC164800.040.45337736
CGTATGC166300.040.3317644
Produced by FastQC (version 0.11.2)