FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_119-3-032817.35100000087095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_119-3-032817.35100000087095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7987208
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC177670.2224431866554621TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24200.035.23672511
GATCGGA26000.032.6395151
CTTAGGC25600.032.3435533
CACACGT26500.032.00863612
ACACGTC26600.031.71913513
ACTCCAG26150.031.66269123
GTATGCC26250.031.02779845
AGCACAC27850.030.86099210
ACGTCTG27150.030.7450915
ACTTAGG27100.030.63615432
GAACTCC27600.030.24400121
CATCTCG27600.029.75581239
GAGCACA29250.029.2300429
AGAGCAC29300.029.180168
CACGTCT28800.028.98365414
TTAGGCA29650.028.0016934
CGTATGC29950.027.2697744
CAGTCAC30950.027.18918827
CACTTAG30300.027.17839231
CGTCTGA31400.026.94217316
Produced by FastQC (version 0.11.2)