FastQCFastQC Report
Fri 7 Apr 2017
HJHJYBCXY_l01n01_119-0-032817.35100000087088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHJYBCXY_l01n01_119-0-032817.35100000087088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6567163
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC485850.7398171782853571TruSeq Adapter, Index 1 (100% over 51bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC92070.14019752517182837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56550.042.5319211
CACACGT57850.041.69282512
GTATGCC57350.041.54630345
GATCGGA58200.041.4261441
ACTCCAG58000.041.15828323
ACACGTC58800.041.01921513
GAACTCC58450.040.99538421
GAGCACA59150.040.928659
AGCACAC59650.040.51013610
ATCACGA59450.040.38303834
ACGTCTG60100.040.09450515
CGTATGC60450.039.49016644
CATCACG60900.039.34674533
CACGATC60850.039.26934436
GTCACAT61850.038.9254629
CAGTCAC61850.038.81632627
CACGTCT62400.038.5806114
GATCTCG62100.038.478939
TCACGAT62300.038.4637235
AGAGCAC63400.038.3979348
Produced by FastQC (version 0.11.2)