FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnz2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnz2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39053027
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2366740.6060324081920717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG172250.022.91310319
AGAGTAC237650.021.73193218
GTACGGG181450.021.67646221
AGTACGG182500.021.64544120
TACGGGG125000.021.61200322
CGCAGAG240250.021.58202215
AGTGGTA268050.019.6134264
ACGCAGA277100.018.79847114
GTGGTAT287950.018.2215755
CAGAGTA294050.017.77477517
GCAGTGG295500.017.7157612
AACGCAG297600.017.60787413
AGCAGTG300500.017.5930081
TCAACGC298050.017.43046811
ATCAACG323650.016.30741510
GCAGAGT326000.015.97949816
TGGTATC341250.015.375446
CAACGCA351650.014.96031712
GTATCAA354800.014.9534538
GGTATCA356300.014.8808187
Produced by FastQC (version 0.11.2)