FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnz1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnz1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26395668
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1647470.6241440830366558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG112550.024.76732619
AGAGTAC152000.023.49141518
AGTACGG122200.022.92462320
GTACGGG122600.022.87892221
CGCAGAG158250.022.65002315
TACGGGG80400.021.71375322
AGTGGTA181850.019.8998574
ACGCAGA181350.019.76438314
AACGCAG190950.018.8972313
GTGGTAT193250.018.796535
CAGAGTA193950.018.57044617
GCAGTGG198600.018.262292
TCAACGC198500.018.16043711
AGCAGTG205700.017.7264751
GCAGAGT208900.017.42284416
TCCGATC97450.016.177076
TGGTATC225550.016.0893486
ATCAACG227350.015.82549710
CAACGCA234100.015.64963812
TACGGGA55500.015.47913822
Produced by FastQC (version 0.11.2)