FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnngnw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnngnw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18886355
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1661010.8794762144415902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA46050.049.8930947
GCCGTAT54500.042.85377548
CCGTATC65300.035.3434349
GGTCGCC67750.034.31838644
GTAGGGA70750.033.4513919
CGTATCA69450.033.3808550
TCGCCGT70100.033.26791846
GTCGCCG70800.032.98650445
TGTAGGG73750.031.90345218
ATCTCGG74250.030.99871336
GTAGATC77700.029.80004532
CTCGGTG77650.029.54570838
GTGTAGG81050.029.49804717
GAGCGTC83250.029.17135410
TAGATCT80150.028.54469533
CGTGTAG84750.028.33174916
TGGTCGC82450.028.28389243
TCTCGGT82050.028.25973737
TCGGTGG87750.026.89093639
GTGGTCG88300.026.68359242
Produced by FastQC (version 0.11.2)