FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnngnw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnngnw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30210449
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1908060.631589421262822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG123400.024.71433319
AGAGTAC169700.023.4196818
CGCAGAG175000.022.78831115
AGTACGG135750.022.31362220
GTACGGG139250.021.72893721
TACGGGG90050.020.42122822
ACGCAGA200450.020.0150514
AGTGGTA205650.019.7777124
AACGCAG215200.018.78747413
GTGGTAT218750.018.5295835
CAGAGTA218150.018.47116917
GCAGTGG222500.018.2239512
AGCAGTG226200.018.089621
TCAACGC225700.017.77561811
CGCCGTA27950.017.65016447
TCCGATC111800.016.5694876
GCAGAGT246900.016.44582616
TGGTATC258700.015.8948476
ATCAACG258500.015.62687610
GTATCAA274550.015.1907778
Produced by FastQC (version 0.11.2)