FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnngn2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnngn2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25036261
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1915560.7651142476905797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA47050.037.6144447
GCCGTAT58950.030.25544748
GTCGCCG69500.025.8107545
GGTCGCC69700.025.73658644
TCGCCGT70200.025.4068246
GTAGGGA71650.025.08589719
CCGTATC71250.024.8387349
GAGTACG117450.024.791219
GTACGGG119500.024.2228721
AGAGTAC156150.024.12618
AGTACGG122600.024.0315420
TACGGGG83700.023.61928722
CGCAGAG160350.023.55810215
TGTAGGG78850.023.0138218
CGTATCA80000.022.55344650
GTGTAGG83550.021.80178817
GTAGATC82100.021.52001432
GAGCGTC86900.021.19778810
ATCTCGG80650.021.09350236
AGTGGTA180150.021.0833444
Produced by FastQC (version 0.11.2)