FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n02_ngnngn1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n02_ngnngn1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27258162
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1686840.6188384968876478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA289890.10634979717267805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG119200.023.53020319
GTACGGG127300.022.16988221
AGAGTAC158350.022.13538218
CGCAGAG160800.022.03331615
TACGGGG86900.021.63810322
AGTACGG130700.021.53941520
CGCCGTA33100.020.94935847
ACGCAGA183900.019.32134614
AGTGGTA186850.018.9981164
CAGAGTA197550.018.00496117
TCAACGC202300.017.5977111
AGCAGTG204800.017.5165441
GCAGTGG206050.017.5005742
AACGCAG208050.017.22779713
GTGGTAT207950.017.2189545
GCAGAGT213600.016.66793616
TCCGATC95750.016.2121456
TGGTATC230950.015.5638316
ATCAACG232950.015.44507310
ACGGGGA67950.015.18227723
Produced by FastQC (version 0.11.2)